Impact of GFP Overexpression on CHO Cell Metabolic Phenotype Alongside a Gene of Interest in an IgG-producing CHO Cell Line
Construction of plasmid to investigate the impact of cumate-inducible overexpression of a gene of interest and green fluorescent protein (GFP) on metabolic efficiency, cell growth, and specific productivity.
Posted by buchanle on Tuesday, April 30th, 2024 in May 2024, Gene overexpression, Green fluorescent protein, Metabolic Engineering, Molecular cloning
Self-regulated Learning and Spelling: A Pilot Study of First Graders
First graders’ self-efficacy and metacognition were measured to assess the development of children’s self-regulated learning abilities.
Posted by buchanle on Tuesday, April 30th, 2024 in May 2024, metacognition, Self-efficacy, Self-regulated learning
Evaluating Cell Culture Systems for Engulfment Receptor Function: Jedi-1 in BV2 and HeLa Cells
Jedi-1 is not expressed endogenously by BV2 cells; transfection of HeLa cells with Jedi-1 induces engulfment of apoptotic neural cells.
Posted by John Lee on Tuesday, May 30th, 2023 in May 2023, HeLa, Jedi-1, microglia, Neuroscience
Recalibration and Spatial Recall in an Immersive Virtual Environment
A study of the effects of age and gender of spatial memory in a virtual environment.
Posted by John Lee on Tuesday, May 30th, 2023 in May 2023, Locomotion, Spatial Memory, Virtual reality
Defining the Immunogenomics of Allopurinol-Induced SJS/TEN
This study aims to validate the involvement of a known HLA risk allele associated with ALP-SJS/TEN as well as to identify other novel HLA alleles or immune factors associated with disease pathogenesis.
Posted by John Lee on Tuesday, May 30th, 2023 in May 2023, Adverse Drug Reaction (ADR), allopurinol (ALP), cytotoxic CD8+ T-cell, HLA Class I alleles, keratinocytes, Severe Cutaneous Adverse Reaction (SCAR), Stevens-Johnson Syndrome / Toxic Epidermal Necrolysis (SJS/TEN), T cell Receptor (TCR)
Breaking Down the Development of Children’s Ordinality Understanding
This paper analyzes the association of various early number skills with the development of numeral ordinality knowledge.
Posted by John Lee on Tuesday, May 30th, 2023 in May 2023, Children, education, Number, Ordinality
Investigating the Effect of Rock Conditions on Ordovician Fossil Properties in Nashville, Tennessee
Fossils were counted and measured in three local outcrops, and these results were compared to the rock conditions in which they were found in order to assess biases in the fossil record.
Posted by John Lee on Saturday, May 7th, 2022 in May 2022, bias, biodiversity, paleobiology, paleoecology, Stratigraphy
Investigating the Differences in Brewing Quality Between Wild and Domestic Yeasts
Through examining the variable fermentation characteristics, the differences between wild and domestic yeasts were determined and analyzed through a commercial brewing perspective.
Posted by John Lee on Saturday, May 7th, 2022 in May 2022, alcoholic fermentation, brewing, microbiology, Yeast domestication, yeasts
The Effects of Daily Activities on Depressive Mood in High School Students
This study examines the relationship between screen use, sleep, and physical activity on mental health in high school students.
Posted by John Lee on Saturday, May 7th, 2022 in May 2022, depressive mood, physical activity, screen time, sleep
The Effect of Background Music on Standardized Math Test Performance in High School Students
Students were given a math test with or without background music in order to measure its effects on test score and completion time.
Posted by John Lee on Saturday, May 7th, 2022 in May 2022, Background music, math test, task performance
An Analysis of the Relationship Between Stunting and Macronu-trient Intakes on a Global Scale
This study examined the relationship between stunting and macronutrient intakes, including animal protein, plant protein, fat, and car-bohydrates in 117 countries.
Posted by John Lee on Saturday, May 7th, 2022 in May 2022, Nutrition, Public Health
Breast Cancer Metastasis to the Bone Microenvironment
A majority of breast cancer patients develop metastases in their bone, and the bone microenvironment plays a large role in recruiting tumor cells from the breast.
Posted by John Lee on Thursday, May 20th, 2021 in May 2021, adipocytes, bone metastasis, breast cancer, stiffness
Liposome-based Strategies to Mitigate Ischemic Stroke-induced Neurodegeneration
Liposome-based drug delivery to the brain is a well-established and an effective approach for delivering neuroprotectants across the BBB in ischemic stroke.
Posted by John Lee on Thursday, May 20th, 2021 in May 2021, Blood Brain Barrier, Drug Delivery Systems, Liposomes, Stroke
Removing Dark Region Artifacts using Deep Neural Networks with a Hyperparameter Search
This study used deep neural networks to remove dark region artifacts in order to create clearer ultrasound images.
Posted by John Lee on Wednesday, May 19th, 2021 in May 2021, Deep Neural Network, Machine learning, Ultrasound
Radiation Emissions of Primordial Black Holes as Dark Matter in a Dwarf Galaxy
Dark matter in a simulated dwarf galaxy was replaced with primordial black holes and the resulting radiation is analyzed.
Posted by John Lee on Thursday, December 24th, 2020 in May 2018, Cosmological Simulations, dark matter, Primordial Black Holes
Analyzing the role of endothelial cell derived Jedi-1 in postnatal neurogenesis
Neural stem cells experience a decrease in quiescence in the absence of Jedi-1 positive endothelial cells.
Posted by John Lee on Wednesday, December 23rd, 2020 in May 2020, Endothelial cells, Jedi-1, neural stem cells, Neurogenesis
Feasibility assessment of heat transfer as an independent variable to compare cold exposure studies of brown adipose tissue
A flow-temperature sensing device was incorporated into a water-blanket system to calculate heat transfer for use as an independent varia-ble in cooling studies
Posted by John Lee on Wednesday, December 23rd, 2020 in May 2020, brown adipose tissue, cold exposure, thermoregulatory physiology
Validating a Dual-Threshold Peak Detection for Calcium Signals Around Laser-Induced Epithelial Wounds
A comparison between an automated method of collection calcium peaks to a manual version to determine the accuracy of the automated system and gain insight into the role of calcium during the wound healing process.
Posted by John Lee on Wednesday, December 23rd, 2020 in May 2020, Calcium Signals, Peaks Detection
Analyzing the Spatial Organization of Tuberous Sclerosis Complex Tumor Cells Using Immunostaining and Computational Approaches
Immunostaining and Computational approaches through the HistoCAT software determined a non-random organization of cells in Subependymal Giant-Cell Astrocytomas (SEGAs), a type of Tuberous Sclerosis Complex tumor.
Posted by John Lee on Wednesday, December 23rd, 2020 in May 2020, HistoCAT, Pax6., Tuberous Sclerosis Complex
Provenance of Zircon in McMurdo Sound Antarctica
This study analyzes the ages of zircon minerals found in the ANDRILL core in order to track past ice flow.
Posted by John Lee on Wednesday, December 23rd, 2020 in May 2020, ANDRILL, Isotope, Provenance, Zircon
Interactive Anatomy and Physiology Augmented Reality Application
An interactive augmented reality prototype application was created as an interactive educational tool for the classroom.
Posted by John Lee on Tuesday, December 22nd, 2020 in May 2019, Augmented reality, education, musculoskeletal, prototype
Utilizing Camera Traps to Analyze Relative Abundance of Basking Turtles in Wetland Ecosystems
Solar irradiance positively influences the time of day at which red-eared sliders bask in wetland ecosystems.
Posted by John Lee on Tuesday, December 22nd, 2020 in May 2019, Biological clocks, habitat degradation, invasive species, physiological responses, thermoregulation
Analyzing homozygous loss of TSC2 mutants in human iPSC-derived neural spheres.
Early neural progenitors with tuberous sclerosis are larger and more disorganized than those without.
Posted by John Lee on Tuesday, December 22nd, 2020 in May 2019, Neurology, stem cell, tuberous sclerosis
Using MicroCT to Improve Precision of Uranium-Thorium Dating in Speleothem Records
Avoiding impurities by using microCT to look below stalagmite surfaces leads to more precise age models and better climate records.
Posted by John Lee on Tuesday, December 22nd, 2020 in May 2019, MicroCT, Speleothems, Uranium-Thorium Dating
Building a Konstanz Information Miner (KNIME) pipeline to assess the utility of immunofluorescence computational analysis of ALCAM in bladder cancer
A KNIME pipeline for performing single-cell, multiplex immunofluorescence
Posted by John Lee on Tuesday, December 22nd, 2020 in May 2018, ALCAM, bladder cancer, KNIME, single-cell analysis
Quantifying Young’s Modulus of Gelatin Substrates for in vitro BBB Models
An examination of the effect of gelatin concentration on Young’s Modulus preformed for future in vitro Blood Brain Barrier studies.
Posted by John Lee on Tuesday, December 22nd, 2020 in May 2018, Blood Brain Barrier, in vitro, Young’s Modulus
Concrete Printing System Construction and Characterization of 3-D Printed Cement-based Materials
Cement-based materials were designed and printed on a novel extrusion system, then characterized.
Posted by John Lee on Tuesday, December 22nd, 2020 in May 2018, 3-D Printing, Additive Manufacturing, Cement, Microstructure, Mix Design
The role of fructose in the expression of extracellular matrix markers and cytokines released by M1 and M2 macrophages that regulate the progression of nonalcoholic fatty liver disease
The role of the sugar fructose on the induction of extracellular matrix proteins and inflammation in polarized macrophages.
Posted by John Lee on Tuesday, December 22nd, 2020 in May 2018, fructose, Macrophages, Nonalcoholic fatty liver disease
Recovery of Putative Temperature-Sensitive Mutations in Coro-navirus Nonstructural Protein 5 Reveals Conserved Determi-nants of nsp5 Structure
This study identifies temperature-sensitive mutations as conserved determinants in coronavirus nsp5 proteins of strains HKU1 and OC43.
Posted by Greta Clinton-Selin on Wednesday, June 28th, 2017 in May 2013, Coronavirus, Nonstructural Protein 5, Temperature-Sensitive Mutations
Using Detrital Zircon Age Distributions to Corroborate Evidence of Early Holocene Megafloods
Zircon grain age distributions provide information on the origins of early holocene megaflood related sediment.
Posted by Greta Clinton-Selin on Thursday, June 8th, 2017 in May 2017, arsenic, Groundwater, Megaflood, Zircon
Development of a Portable and Inexpensive Ultrasound Imaging Device for Use in the Developing World
A portable and inexpensive ultrasound device that could be used in the developing world was developed and tested.
Posted by Greta Clinton-Selin on Thursday, June 8th, 2017 in May 2017, BeagleBone, Phantom, Portable, Ultrasound
Bulk Mechanical Characterization of Nanoparticle Concrete-Equivalent Mortars
Ultimate concrete strength was studied as a factor of both mass and surface area of nanoparticles added to the material.
Posted by Greta Clinton-Selin on Wednesday, June 7th, 2017 in May 2017, compressive strength, concrete, mass, Nanoparticles, nuclear waste storage, surface area
Creating an Agent-Based Model to Explore the Spread of Zika Virus
Predicted Zika outbreak severity directly correlates with human population density.
Posted by Greta Clinton-Selin on Tuesday, May 30th, 2017 in May 2017, Agent-based modeling, Zika Virus
POPDC3 Regulates Epithelial-Mesenchymal-Transition in Colorectal Cancer Cells
The POPDC3 protein was identified as a potential regulator of the epithelial-mesenchymal-transition process in colorectal cancer.
Posted by Greta Clinton-Selin on Wednesday, May 24th, 2017 in May 2017, colorectal cancer, epithelial-mesenchymal transition, Popdc3
Characterization of the Downstream Effects of Apical Mistrafficking of Epiregulin and Potential for Transformation in Polarized Epithelial Cells
Loss of epithelial polarity and mistrafficking of Epiregulin may be a driving force for cancer in polarized epithelial cells.
Posted by Greta Clinton-Selin on Wednesday, June 29th, 2016 in May 2016, Apical, Basolateral, cancer, Epiregulin (EREG), Trafficking
Classification of Infant Cries Using Acoustic Features
Identifying an acoustic feature combination that can accurately differentiate between infant cries.
Posted by Greta Clinton-Selin on Wednesday, June 29th, 2016 in May 2016, classification, cries, features, Matlab, NICU, pain
Determining the Turning Wing Kinematics of a Ruby-Throated Hummingbird
The turning wing kinematics of a ruby-throated hummingbird are determined by use of a blade element model.
Posted by Greta Clinton-Selin on Wednesday, June 8th, 2016 in May 2016, blade element model, computational fluid dynamics, Micro Aerial Vehicles
Improving Patterned Nanoporous Gold as an Effective Surface Enhanced Raman Scattering Template
An imprinted nanoporous gold substrate was optimized to create a low power Raman biological and chemical sensor.
Posted by Greta Clinton-Selin on Tuesday, June 7th, 2016 in May 2016,
Regulation of Pro-Inflammatory Phenotypes by Carcinogenic and Non-Carcinogenic Helicobacter pylori strains
In order to quantify H. pylori virulence, the concentration of IL-8 secreted by AGS gastric epithelial cells co-culture with H. pylori strains, isolated from patients with gastritis or gastric adenocarcinoma was measured and compared.
Posted by Greta Clinton-Selin on Thursday, June 2nd, 2016 in May 2016, gastric cancer, gastritis, Helicobacter pylori, Interleukin-8
Effect of NRIF on p75 Expression
NRIF may be a transcriptional repressor of p75 expression.
Posted by Greta Clinton-Selin on Tuesday, October 20th, 2015 in May 2014, brain, central nervous system, NRIF, p75
IL-6 Secreted from Pancreatic Stellate Cells Activates STAT3 and Promotes Cell Growth and Invasive Ability of Pancreatic Cancer
IL-6 Secreted from Pancreatic Stellate Cells Activates STAT3 and Promotes Cell Growth and Invasive Ability of Pancreatic Cancer
Posted by Printess Berry on Thursday, October 15th, 2015 in May 2014, IL-6 STAT3, Pancreatic ductal adenocarcinoma (PDAC), pancreatic stellate cells (PSCs)
Genetic Diversity Among Mitochondrial Genes of Daucus carota
The quantitative genetic diversity of select mitochondrial genes and population structure of Daucus carota populations was studied.
Posted by Printess Berry on Tuesday, September 15th, 2015 in May 2011, Daucus carota, genetic diversity, Mitochondrial DNA, population structure
The Effects of Tachykinin on Olfactory Reception in Periplaneta americana
A neuropeptide found in invertebrates, the tachykinin-related peptide, caused an overall decrease in olfactory sensitivity for the American cockroach.
Posted by Printess Berry on Tuesday, September 15th, 2015 in May 2011, electroantennogram, olfaction, peptide, Periplaneta americana, Tachykinin, tachykinin-related
The Effect of Pyrvinium, a Potent Small Molecule Wnt Inhibitor, on MSC Biology
Inhibition of the Wnt signaling pathway promotes favorable tissue regeneration and remodeling.
Posted by Printess Berry on Tuesday, September 15th, 2015 in May 2011, Mesenchymal stem cells, pyrvinium, Wnt signaling pathway
Schizophrenia and the Potentiation of the Postauricular Refl ex: A Study on Emotion
This study focused on the emotional perception of schizophrenic patients, using a biological method.
Posted by Printess Berry on Thursday, August 27th, 2015 in May 2012, emotion, postauricular reflex, schizophrenia
Development of an Intelligent, Real-time, Heart Rate Sensitive Virtual Reality System
A real-time analysis of the anxiety level of a subject was predicted by a virtual reality system based on the subject’s heart rate
Posted by Printess Berry on Thursday, August 27th, 2015 in May 2012, Autism programming, heart rate, real-time, Virtual reality
Freestanding Carbon Nanotube Films Fabricated By Post-Electrophoretic Deposition Electrochemical Separation
The development of a novel method for producing buckypapers following electrophoretic deposition of carbon nanotubes is presented.
Posted by Printess Berry on Thursday, August 27th, 2015 in May 2012, Buckypaper, deposition film nanomaterials, electrophoretic, method
Minimizing Carbon Buildup on Graphene Facilitates Electron Transmission
Carbon buildup on graphene hinders transmission of electrons.
Posted by Printess Berry on Thursday, August 27th, 2015 in May 2012, apparatus, carbon buildup, electron transmission thermionic electron, Graphene, LEEPS
Consequences of Altering Transforming Growth Factor Beta Receptor Type III (TβRIII) Expression in the Prostate
The goal of this study was to observe how changing the expression of TβRIII affects prostate cancer progression.
Posted by Printess Berry on Thursday, August 27th, 2015 in May 2012, metastasis, Prostate, receptor, stroma, TGF-β, tumorigenesis
Optimizing Transfection Efficiency and Determining Release Kinetics of Plasmid DNA from Polyurethane Scaff olds in vitro
The purpose of this study was to deliver DNA from biomaterial scaffolds to cells in vitro.
Posted by Printess Berry on Thursday, August 27th, 2015 in May 2012, healing, scaff olds, Skin, transfection
Identification of Novel Helicobacter pylori Adhesins that Bind the Host Cell Receptor Decay-Accelerating Factor (DAF)
H. pylori adherence is critical for gastric cancer development, and this study has identifi ed novel adhesins responsible for DAF-mediated adherence.
Posted by Printess Berry on Thursday, August 27th, 2015 in May 2012, Decay-accelerating factor, gastric cancer, H. pylori