Teachers and collaborators have designed the following activities to accompany the project. They serve as great introductory material to the lab series.
- Pipetting 101: This lab introduces students to the proper use and care of micropipettes. Pipetting Lab (4/16)
- Insects and Their Relatives: This activity introduces students to the characteristics that distinguish insects from their closest relatives and the similarities common to Arthropods. Insects and Relatives Activity
- Wolbachia Project Activity: This simulation of the different labs protocols can be used as an introduction for students or as an overview at professional development workshops. Activity Description; Insect Order Guide
The numbers in parentheses refer to the date (month/year) of the uploaded document.
Lab 1: Insect Identification
Lab 2: DNA Extraction
- Lecture: Molecular Biology Techniques I and II: DNA and PCR (04/17)
- Alternate Protocol: DNA Extraction using Edward’s Buffer
- Alternate Protocol: DNA Extraction using Sigma-Aldrich Extract-and-Amp
- Alternate Protocol: DNA Extraction using Promega Wizard SV Genomic DNA Purification System
- Alternate Protocol: DNA Extraction using the BioRad InstaGene Matrix Kit (#732-6030EDU); Teacher Copy; Student Copy
Lab 3: Polymerase Chain Reaction (Molecular Biology)
- Lecture: PCR – A Review (04/17)
Lab 4: Gel Electrophoresis (Molecular Biology)
- Lecture: Molecular Biology Techniques III: Gel Electrophoresis (08/14)
- Alternate Protocol: DNA Gels using Sodium Boric Acid Buffer
- Alternate Protocol: Fast Blue DNA Stain
- Alternate Protocol: BioRad Gel Electrophoresis classroom study kit (#166-2500EDU).
Lab 5: DNA Sequence Analysis (Bioinformatics)
- Lecture: Bioinformatics Lab – NCBI, BLAST, and Phylogeny (04/17)
- Resources: Using BLAST to determine evolutionary relationships (04/17),
Bioinformatics Exercises, Sea Mammal Nucleotides (.txt file)
Wolbachia In the Encyclopedia of Life
The Encyclopedia of Life (EOL) is a free, online collaborative encyclopedia intended to document all of the 1.8 million living species known to science. It is compiled from existing databases and from contributions by experts and non-experts throughout the world. It aims to build one “infinitely expandable” page for each species, including video, sound, images, graphics, as well as text.
The Microbes Within WebQuest
The Microbes Within leads students on a guided exploration of the alpha-proteobacterium Wolbachia and challenges them to consider the relevance of this obligate, intracellular symbiont with respect to the evolution of species, insect reproduction and human health.
Students are presented with an issue of medical concern and must take advantage of modern-day research to provide persuasive advice to you, the Director of the CDC, through delivery of either a written or oral report. The activity builds upon understanding of basic biology and highlights the impact that one microbe might have on the health of millions worldwide.
Wolbachia In the Classroom
Are you looking for ways to incorporate The Wolbachia Project into your existing curriculum? Would you like to feature the experience as a Summer Course?
Academy of Science Teacher’s Guide
Discover the Microbes Within! faculty member George Wolfe developed this guide to assist teachers in incorporating the Wolbachia labs into the Biology curriculum at the Loudon Co. Academy of Science. It includes connections with other material, relevant background and assessment points.
Acton-Boxborough Regional High School Teaching Materials
Brian Dempsey, assisted by Yuan Kang, taught a summer course based on the Wolbachia lab series at Acton-Boxborough Regional High School, Acton, MA. The syllabus is attached, along with a molecular biology glossary, BioEdit tutorial, powerpoint slides, and assessment questions on dna extraction, pcr and bioinformatics.
- Molecular Bio Lab Glossary
- Using Bioedit
- DNA Extraction questions
- Bioinformatics questions
- PCR questions