Join us to discover the microbes within! The Wolbachia Project is organized as a five-part lab series spanning arthropod collection to DNA extraction, amplification, and sequence analysis. Each Project Guide has been designed to facilitate inquiry-based research experiences and optimize student success in the classroom. Unless otherwise specified, all material is available under the CC BY-NC-ND 4.0 license.
- Lab 1: Insect Identification
- Lab 2: DNA Extraction
- Lab 3: Polymerase Chain Reaction
- Lab 4: Gel Electrophoresis
- Lab 5: Bioinformatics
To get started, we recommend that you:
- Review materials list for each lab to ensure you have necessary equipment. If you require equipment, review our Loaner Program.
- Contact us to obtain insect controls (Wolbachia-infected and Wolbachia-uninfected), a Wolbachia (+) DNA control, and PCR primers.
- Download the list of Next Generation Science Standards aligned with this project.
- Download the journal article “Using the Wolbachia Bacterial Symbiont to Teach Inquiry-Based Science: A High School Laboratory Series.”
In some cases, teachers and collaborators have provided alternate protocols to accommodate availability of classroom resources. These resources, as well as previous versions of the labs, are available in the Resource Library.
Free Loaner Program
Are you interested in The Wolbachia Project but don’t have access to equipment or costly reagents? The Free Loaner Program will provide everything you need to get started!