Cryo-EM Project Support – Getting Started
Starting a CSB Cryo-EM Project
The CSB Cryo-EM Facility offers training and consultation in planning EM experiments, sample preparation and imaging, using equipment and data analysis.
Before pursuing cryo-EM as an analytical technique, one must have a biochemically pure sample. Sample purity must be improved in each investigator’s laboratory. We recommend discussing the project with our staff to build the final-phases of the purification protocol, since some buffers are not compatible with cryo-EM.
Visit the facility iLab site to complete a training or consultation request to discuss your goals with facility staff. Be prepared to discuss research plan by providing information on sample quality, complex size, stability and known structural information.
Information to set up an iLab Account: iLab Account Basics and Setting Up a New User Account Guide.
If you are new to structural biology research or require more assistance beyond preparing grids and using the microscopes, submit a CSB Consultation form for CSB staff scientists to assess how the available CSB facilities can aid your projects.
CSB Cryo-EM Training
The CSB Cryo-EM Facility is a shared resource for the Center of Structural Biology (CSB). All users share responsibility for careful, error-free usage of the microscopes and other equipment in the facility. Facility usage can be accessed via facility staff, unless the user is a facility-certified microscopist. Microscopists must be trained or certified by the facility director. All facility usage must be logged and authorized.
Titan Krios Access
To request access to the Titan Krios, visit the facility iLab site and complete the Krios Request for Screening and Data Acquisition from under the Request Services tab.
EM Tools Kits and Vendors
The CSB Cryo-EM Facility provides tools and supplies for common use. Regular facility users are advised to stock and maintain their own tool kits.
Common EM supply vendors: