| Bioapplication Analysis | Function | Uses | Applications |
|---|
| Cell Cycle | Multiparameter assay that automatically classifies cell cycle phase based on DNA content, and correlates levels of up to three related targets at both the single cell and well population levels. | Flexible application for screening of effectors of cell cycle progression, target validation, general life science research, and characterization and quality control of cell types. | Cancer, Cell Signaling, Basic Research |
| Cell Health | Enables examination of a variety of cell health markers including cell viability, cytotoxicity, and apoptosis. Applies advanced cell-level logic functions for discriminating between Early, Middle, or Late toxicity stages. | Generate critical toxicology information to aid in the identification, selection, and prioritization of potential therapeutic candidates. Provide relevant, high content information for more reliable insight into the potential toxicity of drug candidates. Narrow field of drug candidates to undergo animal testing to the most positive compounds. Reveal mechanistic information critical to understanding toxic effects. | Toxicology, Cell Survival, Cancer |
| Cell Motility | Quantifies the area of the track produced by motile cells on a surface (2D) of fluorescent beads. | Analysis of cellular motility in a fixed endpoint assay, as opposed to vector tracing of living cells. Also useful for profiling cancer cell lines by assessing compounds that inhibit motility or metastasis. | Cancer, Cell Signaling, Inflammation, Wound Healing, Cellular Profiling, Angiogenesis, Vascular Disease, Other |
| Cell Spreading | Measures shape and size of cells and colonies. | Analysis of cellular shape change during attachement, motility, or differentiation. Successfully used to analyze differential adhesion properties on various matrices. Also allows the user to measure colony formation and nuclear count. | Cancer, Cell Signaling, Cellular Profiling |
| Colocalization | Enables the measurement within multiple cellular regions for the presence or coincidence of fluorescent targets. | Quantifies the dregree of colocalization of a target of interest (often a protein) with other proteins, organelles, subcellular compartments. | Protein-protein interactions, Internalization, Intracellular Trafficking, Cytoskeletal Protein Formation/Disruption |
| Comets | Enables detection of genotoxicity and DNA damage by the automated quantitation of comets (electrophoresed nuclei). Measured features include the %DNA in Tail and Olive Tail Movement for determining DNA damage potential of compounds. Can be combined with FISH analysis using other stains in additional channels. | Screen compounds for genotoxicity earlier in the drug discovery process; get better information during lead optimization and SAR studies. | Cytotoxicity, Genotoxicity, Toxicity Profiling, Cancer |
| Compartmental Analysis | General purpose application with a high level of flexibility for analysis of a wide range of biological assays. | Intensity analysis including ratiometric intensity analysis, translocation analysis, and detection of discrete cellular spots and objects in different compartments. | Pathway analysis, Cellular Signaling, Proliferation and Cell Death (PCD), Endocrine Profiling, Drug-Induced Liver Injury (DILI), DNA Damage, Others |
| Cytoplasm to Cell Membrane Translocation | Specifically measures target translocation between cytoplasm and cell membrane. | Unique application for analysis of targets such as PKC, GLUT-4, and ras. Also useful for comparison of receptor ratio between the cell surface interior. | Cancer, Cell Signaling, Metabolic Disease, Other |
| Cytoplasm to Nucleus Translocation | Patented method to monitor target translocation between two sub-cellular regions. | Analysis of nuclear translocation for kinases and transcription factors, simple intensity measurements within the cell, determination of transfection efficiency, and analysis of nuclear or cytoplasmic protein levels. | Cancer, Cardiovascular, Inflammation, Neurobiology, Cell Signaling, Infectious Disease, Others |
| Micronucleus | Enables detection of genotoxicity through examining the frequency of micronucleus formation in single cells. Also reports early cytotoxicity (viability, cell cycle delay through %cytostasis), frequency of mononucleate and late-stage toxicity (permeabilized cells). | Screen compounds for genotoxicity earlier in the drug discovery process; get better information during lead optimization and SAR studies. | Cytotoxicity, Genotoxicity, Toxicity Profiling, Cancer |
| Molecular Translocation | Multiparameter assay to measure and correlate multiple intracellular translocation events at the single cell level. | Pathway analysis, kinase specific studies, transcription factor activity profiling. | Multiple Target Translocation, Others |
| Morphology Explorer | Detects and quantifies multi-cellular, cellular, sub-cellular morphological changes. Characterizes and measures cell proximity, cell colonies, process outgrowths, distribution and organization of intracellular fluorescent structures such as cytoskeletal fibers and discrete objects such as organelles. Does 1st order (intensity dependent) and 2nd order (intensity independent) texture analysis of intracellular stains. | Includes analysis of colonies, cytoskeletal rearrangement, tube formation, intracellular protein distribution, cell shape and size changes, and process outgrowth. | Intracellular Morphology, Whole Cell Morphology, Inter/Multi-Cellular Morphology, Myoblast Formation, Cytoskeletal Rearrangement, Colony Formation, Tubes, Multi-Nucleated Cells, Functional Genomics, Cancer, Phenotypic Characterization, Neuroscience |
| Multiparameter Cytotoxicity | Measures multiple cytotoxicity-related intensity and shape-based outputs in a single assay: nuclear morphology, cell permeability, cell count and either lysosomal pH or mitochondrial transmembrane potential. | Profiling multiple aspects of complex cytotoxicity and apoptosis pathways. Monitoring multiple aspects of cell life/death. | Cytotoxicity Profiling, Cell Survival, Cancer, Nuclear Size/Morphology Changes, Cell Membrane Permeability, Organelle Function |
| Neuronal Profiling | Enables automated quantitation and correlation of neuronal and neurite morphology along with synaptogenesis. Analysis can range from simple neurite outgrowth analysis to complexity analysis of extended neurite outgrowth and branching, to correlative studies on synaptic function, determined by the colocalization of pre-and post-synaptic markers, all within one software package. | A non-biased, automated, and validated method to investigate drug therapies for modulating neuronal growth and/or neurotransmission. | Neurite Detection, Synaptogenesis, Neurotoxicity Profiling, Paralysis, Alzheimer’s and Neurodegenerative Diseases, Depression, Epilepsy, Developmental Neurotoxicity |
| Organelle Health | Enables examination of a variety of cell health markers including cell viability, cytotoxicity, and apoptosis. Applies advanced cell-level logic functions for discriminating between Early, Middle, or Late toxicity stages. | Generate critical toxicology information to aid in the identification, selection, and prioritization of potential therapeutic candidates. Narrow field of drug candidates to undergo animal testing to the most promising compounds. Reveal mechanistic information critical to understanding toxic effects. | Toxicology, Cell Survival, Cancer |
| Spot Detector | Rapid yet flexible assay that measures “spots” representing internalized receptors or other discrete punctate targets in up to four channels. | | Receptor Internalization, Beta-Arrestin Redistribution, Organelle Analysis (Lysosome, endosome, mitochondria, Golgi), Lipid Accumulation, Phospholipidosis, Cell Counting, Proliferation, Rare Event Analysis, Cell Viability, Mitotic- and S-phase Indexing, Cancer, Cell Signaling, GPCRs, Metabolic Disease, Others |
| Target Activation | General purpose assay for measurement of average and total intensity within a user-defined cellular domain in each of 6 channels. | Incl. rare event analysis, population analysis, gene expression/knockdown, expression profiling. | Cell Viability, Mitotic Index, Others |
| Tube Formation | Measures degree of angiogenic tube formation by endothelial cells. Reports tube areas and lengths, node count, angiogenic index. | Analysis of conditions or factors that promote or inhibit angiogenesis. Screening for therapeutic compounds. | Cancer, Cell Signaling, Angiogenesis, Differentiation, Toxicity |
| Kinectics | Versatile time-lapse assay to measure 2D displacement, distance travelled, length, speed, directionality, angle change, turn strength, spatial persistence, acceleration, net displacement, total distance, drift speed, temporal persistence. | Kinetic measurements of any cell/well feature vs. time curve. Motility measurements of position vs. time trace for each cell. Statistical summary of cell motility on a per-well level. | Chemotaxis, Haptotaxis, Cancer, Wound Healing, Cell Dispersion, Invasion, Motility |
| Zebrafish Toxicology | Quantifies morphological changes in 1-5 day Zebrafish embryos due to toxic stimuli. Images can be acquired in either brightfield or fluorescence. Additional fluorescence channels are used to measure spot formation and other biomarkers. | Automated small animal toxicity screen to supplement in vitro cell-based toxicity studies. | Zebrafish, Cytotoxicity, Genotoxicity, Toxicity Profiling, Development |
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